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pwallium dcas9 vpr  (Addgene inc)


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    Structured Review

    Addgene inc pwallium dcas9 vpr
    Pwallium Dcas9 Vpr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pwallium+dcas9+vpr/pWalium20-10XUAS-3XFLAG-dCas9-VPR+(Plasmid+%2378897)/pmc08744452-239-0-7
    Average 93 stars, based on 16 article reviews
    pwallium dcas9 vpr - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Cloning:

    Article Title: Base Editing of Somatic Cells Using CRISPR–Cas9 in Drosophila
    Article Snippet: .. Cloning of pattB-Actin5C-BE2(pActin5c-CD-dCas9m4-UDI-attB), Addgene# 104879 pWallium-dCas9-VPR (containing Homo sapien codon optimized dCas9m4; Addgene# 78897 19 ) was digested with Nde I/ Eco RI, and a 3.3 kB fragment isolated. .. This fragment was used to replace the Nde I/ Eco RI fragment from pCMV-BE2 (Addgene# 73020 18 ) digested with Nde I and Eco RI using a three-way ligation reaction (Rapid DNA ligation kit; Roche).

    Isolation:

    Article Title: Base Editing of Somatic Cells Using CRISPR–Cas9 in Drosophila
    Article Snippet: .. Cloning of pattB-Actin5C-BE2(pActin5c-CD-dCas9m4-UDI-attB), Addgene# 104879 pWallium-dCas9-VPR (containing Homo sapien codon optimized dCas9m4; Addgene# 78897 19 ) was digested with Nde I/ Eco RI, and a 3.3 kB fragment isolated. .. This fragment was used to replace the Nde I/ Eco RI fragment from pCMV-BE2 (Addgene# 73020 18 ) digested with Nde I and Eco RI using a three-way ligation reaction (Rapid DNA ligation kit; Roche).

    Article Title: Base Editing of Somatic Cells Using CRISPR–Cas9 in Drosophila
    Article Snippet: .. pWallium-dCas9-VPR (containing Homo sapien codon optimized dCas9m4; Addgene# 78897 19 ) was digested with Nde I/ Eco RI, and a 3.3 kB fragment isolated. .. This fragment was used to replace the Nde I/ Eco RI fragment from pCMV-BE2 (Addgene# 73020 18 ) digested with Nde I and Eco RI using a three-way ligation reaction (Rapid DNA ligation kit; Roche).

    Article Title: Base editing using CRISPR/Cas9 in Drosophila
    Article Snippet: .. pWallium-dCas9-VPR (containing homo sapien codon optimized dCas9m4; Addgene# 78897 ( )) was digested with NdeI / EcoRI , and a 3.3 kB fragment isolated. .. This fragment was used to replace the NdeI / EcoRI fragment from pCMV-BE2 (Addgene#73020 ( )) digested with NdeI and EcoRI using a 3-way ligation reaction (Rapid DNA ligation kit, Roche).



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    93
    Addgene inc pwallium dcas9 vpr
    Pwallium Dcas9 Vpr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pwallium+dcas9+vpr/pWalium20-10XUAS-3XFLAG-dCas9-VPR+(Plasmid+%2378897)/pmc08744452-239-0-7
    Average 93 stars, based on 1 article reviews
    pwallium dcas9 vpr - by Bioz Stars, 2026-10
    93/100 stars
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